We have demonstrated that donor specific DNAm patterns are

2018-10-20

We have demonstrated that donor-specific DNAm patterns are maintained upon reprogramming into iPSCs (Shao et al., 2013), and these also remain upon redifferentiation into iPS-MSCs. Thus, there is some epigenetic memory after chromatin remodeling—whether these donor-specific DNAm patterns are functio

br Results We investigated the identity

2018-10-20

Results We investigated the identity of human NPCs extracted from the foreskin dermis through fluorescence-activated cell sorting (FACS)-based isolation of cell subpopulations and subsequent characterization of the sorted chemokine receptor (Figure 1). Immunofluorescent and flow-cytometry analys

Based on their well documented activity

2018-10-20

Based on their well-documented activity profile, we employed M-CSF and G-CSF to direct terminal myeloid differentiation toward the monocyte/macrophage or granulocyte lineage in our studies. We found that the IL-3/M-CSF combination efficiently promoted the differentiation of iPSCs into iPSC-MΦ that r

br Results br Discussion We

2018-10-20

Results Discussion We have established a novel serum-free culture method for generating and expanding CD271+CD73+SOX9+ chondrogenic ectomesenchymal trpv1 from hPSC-derived, CD271hiCD73− neural crest-like progeny. The differentiation pathways from hPSCs to chondrocytes via chondrogenic ectomes

Ectopic BMP signaling activates the primitive erythroid prog

2018-10-20

Ectopic BMP signaling activates the primitive erythroid program, while inhibiting the pathway blocks ventral blood island formation in Xenopus (Zhang and Evans, 1996). This suggests that BMP signaling may represent a critical influence on erythroid differentiation, in addition to its role in mesoder

br Introduction Glioblastoma GBM is the most

2018-10-20

Introduction Glioblastoma (GBM) is the most common primary malignant TH-302 tumor in adults. Despite multimodal aggressive therapies, survival of a vast majority of the patients is less than 2 years with the 5-year survival rate as low as 5% (Stupp et al., 2009). Previous efforts in the developme

br Disclosure of potential conflicts of interest br

2018-10-20

Disclosure of potential conflicts of interest Acknowledgements Introduction In many cells, the plasma membrane and underlying topotecan Supplier filaments interact dynamically with each other to produce cell surface blebs, which may be either dynamic (non-apoptotic) or apoptotic (Weng, 2016

All PGCs differentiated from ESCs

2018-10-20

All PGCs differentiated from ESCs in our study, regardless of differentiation approach or sorting strategy, were capable of implementing ICC erasure at the Snprn ICC. However, unlike E9.5 PGCs from the embryo, the hypomethylated state at this ICC was unstable, and in every case re-methylation was in

We next aimed to characterize changes in

2018-10-20

We next aimed to characterize changes in huSCs during ex vivo culture, which, in mice, results in a marked reduction in the capacity for tissue regeneration and self-renewal upon subsequent transplantation (Gilbert et al., 2010; Montarras et al., 2005). For this analysis, we used a basal culture med

br Results br Discussion In this study we provide

2018-10-20

Results Discussion In this study we provide several lines of evidence that Dnd serves as a critical PGC specifier in medaka. First, dnd knockdown is capable of specifically and completely abolishing PGC formation and its overexpression is sufficiently capable of inducing ectopic PGCs in embry

Zscan is sometimes considered as a marker of

2018-10-20

Zscan4 is sometimes considered as a marker of highly pluripotent status, merely due to its expression at the two-cell stage during development (Cerulo et al., 2014). However, based on our observation, we would like to propose that the expression of Zscan4 is activated in response to telomere shorten

br Experimental Procedures br Author Contributions br Acknow

2018-10-20

Experimental Procedures Author Contributions Acknowledgments B.Z. was supported by an NYSTEM post-doctoral training fellowship, The SKI Stem Cell Research Facility is supported by NYSTEM grants C029153 and C024175 and The Starr Foundation. The work was further supported in part by NIH/NCI g

br Experimental Procedures br Introduction Cells have been

2018-10-20

Experimental Procedures Introduction β Cells have been reported to have the potential to proliferate after experimental ablation, such as chemical treatment or partial pancreatectomy (Bonner-Weir et al., 2010) (Cano et al., 2008; Nir et al., 2007; Xu et al., 2008). Although controversial, β ol

Like any other method karyotyping cannot prove the absence o

2018-10-20

Like any other method, karyotyping cannot prove the absence of variant cells. Nonetheless, mosaicism can be excluded to a certain degree by analyzing an appropriate number of metaphases (Hook, 1977). The exact number of metaphases scored may depend on the ultimate application of cells. For example,

Hello world!

2018-07-29