Proteins from lysenin family were detected by Western

Proteins from lysenin family were detected by Western blotting (WB) in coelomocyte-containing coelomic fluid of 9 adult earthworms, i.e. the 3 specimens (1–3) from the each group of earthworms, Ea, EfM−, EfM+, as described in [1]. The chemiluminescent signals were observed at the molecular weights 35–39kDa; single (in Ea1) or double (in all remaining samples) WB bands were noticeable in all E. andrei and E. fetida specimens (see Fig. 3A in [1]). SDS-PAGE bands corresponding to molecular weight of WB bands (see Fig. 3B in [1]) were subjected to LC-MS/MS analysis.
Acknowledgements The mass spectrometry measurements were done with the equipment spectrometer Q-Exactive purchased by the European Regional Development Fund in the framework of the Polish Innovation Economy Operational Program (contract No. POIG.02.01.00-12-167/08, project Małopolska Center of Biotechnology). This work was supported by the grants from National Science Center, Krakow, Poland (UMO-2012/05/B/NZ4/02428 - for SKK, and B/NZ4/01640 for BP), and K/ZDS/005405 (for BP). Faculty of Biochemistry, Biophysics and Biotechnology azilsartan medoxomil a partner of the Leading National Research Center (KNOW) supported by Ministry of Science and Higher Education, Poland.
Data The data presented in this article show the morphological and ultrastructural characteristics of psychrotolerant bacterium Shewanella olleyana sp. nov. obtained by TEM. Cells were grown and prepared following an optimized procedure. S. olleyana cells were grown and propagated in solid medium at temperatures between 4 and 10°C for 24–48h. Complete sedimentation of suspended S. olleyana cells (grown in solid medium) was achieved following centrifugation at 4000 x g for 10min at 4°C. The presence of residual or contaminating reduced form of iron and/or iron sulphide was detected when cells are grown in liquid medium.
Experimental design, materials and methods
Acknowledgements This work was supported by the Philippine Nuclear Research Institute, Department of Science and Technology (PNRI-DOST) and the Institute of Biology, College of Science, University of the Philippines. We would like to thank Dr. Preciosa Corazon Pabroa and Mr. Joseph Michael Racho for their assistance in the XRF spectroscopy analysis.